Cell Surface Expression of α1D-Adrenergic Receptors Is Controlled by Heterodimerization with α1B-Adrenergic Receptors

Cell Surface Expression of 1 D - Adrenergic Receptors Is Controlled by Heterodimerization with 1 B - Adrenergic Receptors

1-Adrenergic receptors (ARs) belong to the large Class I G protein-coupled receptor superfamily and comprise three subtypes ( 1A, 1B, and 1D). Previous work with heterologously expressed C-terminal green fluorescent protein (GFP)-tagged 1-ARs showed that 1Aand 1B-ARs localize to the plasma membrane, whereas 1D-ARs accumulate intracellularly. We recently showed that 1Dand 1B-ARs form heterodimer...

2C-Adrenergic Receptors Exhibit Enhanced Surface Expression and Signaling upon Association with 2-Adrenergic Receptors

The 2C-adrenergic receptor ( 2CAR) is known to be poorly trafficked to the cell surface when expressed in a variety of cell types. We tested the hypothesis that the surface expression and signaling of 2CAR might be enhanced by heterodimerization with other G protein-coupled receptors (GPCRs). Cotransfection of 2CAR with more than 25 related GPCRs revealed that only coexpression with the 2-adren...

Morphology of astroglial cells is controlled by beta-adrenergic receptors

Astroglial cells in vivo and in vitro respond to hormones, growth factors, and neurotransmitters by changing from an epithelial-like to stellate morphology. We have studied the temporal relationship between receptor activation, second messenger mobilization, and morphological changes using LRM55 astroglial cells. Maintenance of an altered morphology required continuous beta-adrenergic receptor ...

Dendritic Cell Migration Controlled by a1b-Adrenergic Receptors

Heterodimerization of 2 A - and 1 - Adrenergic Receptors

and 2–adrenergic receptors are known to exhibit substantial cross-talk and mutual regulation in tissues where they are expressed together. We have found that the 1-adrenergic receptor ( 1AR) and 2A-adrenergic receptor ( 2AAR) heterodimerize when coexpressed in cells. Immunoprecipitation studies with differentially tagged 1AR and 2AAR expressed in HEK-293 cells revealed robust co-immunoprecipita...